Epistasis analysis is one of the most direct methods for defining functional relationships between genes and proteins. These interactions can be negative (synthetic lethality) or positive (suppression). Whole-genome high-throughput epistatic maps (E-MAP) were peviously published for S. cerevisiae; here, the authors focus on S. pombe. E-MAPs are generated through generating pairwise knock-outs and assaying their henotypes (usually growth in complex media), comparing them to the single-gene mutants. This E-map includes ~118,000 double mutants in 550 genes invloved in different aspects of cellular processes. In this set, similar to previous E-MAPs, the correlation between protein-protein interactions (PPI) and epistasis scores is apparent (see the figure below from the original paper).
The authors have also focused a great deal on dissecting the RNAi machinery in S. pombe. This study resulted in the identification of a novel component in this machinary (rsh1).
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