The authors measured transcript abundance between BY4716 and RM11-1a yeast strains as well as their segregants to do QTL mapping. Over half the transcripts linked to a QTL, but extensive modeling showed that most traits would have multiple loci and no single locus would have a large effect. The authors limited the transcripts they looked at based on heritability scores. They also did a breakdown of inheritance patterns for the phenotypes and concluded that an overwhelming proportion are inherited in a transgressive segregation manner as compared to an additive effect. A small but nontrivial were found to be epistatically inherited, and most were in the transgressive segregation camp.
Other notes:
-median heritability was 27%
-no QTLs detected for nearly 40% of highly heritable transcripts
-only 3% of highly heritable transcripts have single-locus inheritance, 17-18% for one or two loci, over half for at least five loci
-11% had directional model (phenotype of one parent)
-59% were transgressive segregation (outside parental range)
-16% epistatic (tests difference between means of segregants and parents)
-“opposing QTLs may be a mechanism for generating diversity in subsequent generations”
Left: Directional; Center: Transgressive; Right: Epistatic
Damn you genetic complexity!
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