The strict linkage between the genes on a chromosome is broken through recombination. In addition to its evolutionary significance, meiosis crossovers also facilitate chromosome segregation. Gene conversion happens either through crossovers (which involves reciprocal exchange of strands) or non-crossover events (through synthesis-dependent strand annealing). In this paper, the authors have presented a high-resolution map of these events. The method (shown in the figure below) involves high-resolution genotyping of all the four viable spores from the meiosis event.
Then, they use the genotyping data to find both crossover and non-crossover conversions (Part b of the figure above from the original paper). The resulting map can be probed for the identification of recombination hotspots. The reported hotspots in this study include almost all of the previously known regions. Interestingly, crossover and non-crossover events have distince distributions with different hotspots.
The authors also test recombination methods to elucidate the role of different pathways in the obsereved deregulations. In the end, they also make an interesting case for interference and how crossover and non-crossover events also show spatial avoidance.
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